Polymers technology demonstrates effective non-viral DNA delivery for in vivo cardiac study


Techulon announced that its technology was used successfully in a recent in vivo study focused on blocking downstream inflammatory pathways. Techulon’s polymers were used as delivery agents for an NF-κB oligodeoxynucleotide (ODN) decoy used to limit myocardial infarction during an ischemic-repurfusion event simulating a heart attack. 

This study compared the efficacy and toxicity of poly(glycoamidoamine)s (PGAA’s) against well known lipid and polyethyleneimine technologies for delivery of the decoy to H9c2 cells and cardiomyocytes in vitro, and to the in vivo murine heart.

The PGAA’s demonstrated superior delivery efficacy and significantly reduced toxicity over the reference delivery modes.

The paper, entitled “In vivo delivery of nucleic acids via glycopolymer vehicles affords therapeutic infarct size reduction in vivo,” was authored by Tranter, Liu, He, Gulick, Ren, Robbins, Jones and Reineke and appeared in the 20 December issue of Molecular Therapy.

“This study demonstrates that the PGAA polymers enabled low toxicity delivery in vivo. The most significant finding in the study was that this composition provides for much higher DNA delivery to cell nuclei in vivo than what was predicted by in vitro comparisons of the PGAAs to lipids and polyethyleneimines,” said Joshua Bryson, principal scientist at Techulon. “This compelling study demonstrates the PGAAs to be an efficacious delivery tool offering a non-toxic vehicle for therapeutics companies.”